Preparation of technetium-99m labeled ibuprofen by direct route and technetium-99m tricarbonyl route: a comparison of in vivo behaviors

Abstract

In the current study, ibuprofen (ibu) which is a non-steroidal anti-inflammatory drug (NSAID) was radiolabeled with ^99mTc using two different methods: stannous chloride method (direct route) and technetium-99m tricarbonyl [^99mTc(CO)₃]⁺ route. Thus, it’s aimed to investigate the radiolabeling potential of ibu for inflammation detection and to monitor if there is any difference in in vivo distribution depending on the radiolabeling route. Quality control studies of both radiolabeled ibu were performed by radiochromatographic methods (Thin Layer Liquid Radio Chromatography and High Performance Liquid Radio Chromatography). Radiolabeling yields of ^99mTc-ibu and ^99mTc(CO)₃-ibu were determined as 99.05 ± 0.83% and 91.79 ± 3.30% (n = 5), respectively. Experimental lipophilicities of both radiolabeled ibu were determined. The biological behavior of both radiolabeled ibu was investigated in healthy Albino Wistar male rats by in vivo biodistribution studies. It was seen that both radiolabeled ibuprofen showed renal excretion while organ uptakes of ^99mTc-ibu and ^99mTc(CO)₃-ibu differ against time.

Keywords:

• Ibuprofen
• Technetium-99m
• radiolabeling
• in vivo biodistribution
• NSAID

Disclosure statement

The authors report no declarations of interest.

Additional information

Funding

Present study was financially supported by the Ege University Scientific Research Project numbered 2011 NBE 010 (Bornova-Izmir, Turkey).