Design and fabrication of Sesamol-loaded transfersomal gel for wound healing: physicochemical characterization and in-vivo evaluation

Abstract

Objective

In the present study, an attempt has been made to develop SL-loaded transfersomal gel for the effective treatment of delayed wound healing.

Significance

The wound healing process consists of a complex series of biochemical events and changes in cellular activity that restore the integrity of the skin and the subcutaneous tissue. Sesamol (SL), which is a natural phenolic compound, is known for its antioxidant properties, anti-inflammatory properties, and wound-healing abilities.

Methods

A thin-film hydration method was used to prepare SL-loaded transfersomes. Different formulations containing Tween-80 and Span-80 as edge activators were prepared and optimized. Various characteristics of vesicles were assessed, such as size, shape, loading efficiency, deformability, and in vitro skin penetration. The optimized formulation was then incorporated into 1% carbopol 940 gel. An in vivo wound healing potential of the selected formulation was assessed by an excision wound model.

Results

The SL-loaded transfersomal gel displayed improved skin penetration and better skin deposition. Wound healing studies showed that the highest wound contraction was observed with SL-loaded transfersomes. Following 21 days of application of the transfersomal gel, a marked improvement in skin histological architecture was found.

Conclusion

The study findings suggest that transfersomal gel has great potential as a therapeutic option in wound healing.

Graphical Abstract

Keywords:

• Sesamol
• delayed wound healing
• topical delivery
• transfersomal gel
• physicochemical characterization
• wound healing activity

Ethics approval and consent to participate

The study was approved by the Faculty of Pharmacy, Integral University, Lucknow, India (CPCSEA approval#-IU/IAEC/19/17). All animal experiments were performed strictly in humane as possible and complied with the guidelines for animal care with the approval of the Institutional Animal Ethical Committee.

Human and animal rights

No humans were used in this study. All animal experiments performed were in accordance with protocols evaluated and approved by the Institutional Animal Ethical Committee (IAEC).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability materials

The authors confirm that the data supporting the findings of this study are available within the article.

Additional information

Funding

The authors are thankful to the Faculty of Pharmacy, Integral University, for providing all necessary facilities related to the present work [Manuscript Communication Number: IU/R &D/2021-MCN0001305].