Evaluation of chitosan and alginate immobilized Methylobacterium oryzae CBMB20 on tomato plant growth

ABSTRACT

Methylobacterium oryzae CBMB20, a promising plant growth promoting bacteria (PGPB) and a biocontrol agent, was immobilized in different formulations such as wet chitosan, dry chitosan, wet alginate and dry alginate and were tested for tomato plant growth promotion. Chitosan solution (1.5%) with pH 5.5–6.0 and 90 min contact time was found optimal for immobilization. The chitosan formulations showed better entrapment efficiency and good degradability resistance apart from slow release of cells under prolonged incubation. Survivability of bacteria (80%) was observed in wet chitosan formulation even after 90 days of storage at 4°C. The spermosphere survival of bacteria was high in both dry and wet chitosan formulations applied soils even after 21 days under greenhouse conditions. While the alginate formulation degraded fully, partial degradation of chitosan formulation was observed even after 30 days, indicating its ability to support the survival of M. oryzae CBMB20 in soil. Plants inoculated with wet chitosan formulation registered 1.3 fold increase in the shoot and root length and dry weight compared to other treatments. Hence, chitosan formulation supporting better plant growth compared to alginate will be a better carrier for taking bacteria to the plant rhizosphere and thereby promote plant growth.

KEYWORDS:

• Methylobacterium oryzae CBMB20
• immobilization
• spermosphere survival
• entrapment efficiency
• degradation

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by Basic Science Research Program, National Research Foundation (NRF), Ministry of Education, Science and Technology [2015R1A2A1A05001885], Republic of Korea