Morphological and molecular characterization of arbuscular mycorrhizal fungal communities inhabiting the roots and the soil of saffron (Crocus sativus L.) under different agricultural management practices

ABSTRACT

Crocus sativus L. cultivation is expanding to areas with low soil fertility, where mycorrhizal fungi are supposed to be essential for plants growth and ecosystems functioning. Agricultural practices applied under these conditions should lead to good saffron productivity and quality. Our objective was to study the density and diversity of mycorrhizal fungi populations associated with saffron grown in Taliouine (Morocco) under different agricultural management practices (fertilization type, age and plantation method). Morpho-anatomical studies identified rhizospheric mycorrhizal spores and assessed root colonization by arbuscular mycorrhizal fungi (AMF). Molecular identification of AMF was realized by sequencing the Large Subunit (LSU) rDNA gene region. Among the eleven species of AMF spores identified, Funneliformis and Rhizoglomus species were the most abundant (> 35%). Modern saffron plantation showed higher roots colonization rates (mycorrhization intensity (100%) and frequency (51.6%)), while in traditional plantations lower mycorrhization frequency values were found (17.4%). LSU sequencing identified five AMF genera and three unknown genomic groups, whereas Shannon diversity index indicated that AMF community composition changed significantly according to plantation age and fertilization type. Our results contribute to a better knowledge of saffron AMF communities and open new perspectives for a rational utilization of the agricultural practices for organic saffron production.

KEYWORDS:

• Arbuscular mycorrhizal fungi
• Crocus sativus L.
• roots colonization
• morpho-anatomical diversity
• molecular identification

Acknowledgments

The authors thank the responsible of the company 123 SAFRAN for giving them the opportunity to sample under the different agricultural practices used for saffron cultivation in their farm, the financial support to the PhD student Imane Chamkhi and for all the logistic, technicians and workers that they put at their service during the sampling.

Disclosure statement

No potential conflict of interest was reported by the authors.