FTIR spectroscopy in biomedical research: how to get the most out of its potential

Abstract

Fourier Transform Infrared (FTIR) Spectroscopy, in particular ATR-FTIR, is a widely used technique that allows, in a very short time, to screen biological samples and to identify its specific spectral signature, being an important tool for clinical diagnosis and biomarker discovery. FTIR spectroscopy is used to screen cells, tissues and biofluids and is already implemented in biomedicine, mainly in pre-clinical setting. Although the experimental procedure is easy to implement, sample preparation, definition of spectra acquisition parameters and spectral analysis are crucial steps to obtain reliable and reproducible results. However, the selection of experimental conditions for spectral analysis can be a difficult task for researchers because the information is dispersed and often the choice is made in an empirical and inaccurate way. This review gathers and summarizes studies using FTIR in pre-clinical and clinical setting and aims to systematize information and propose some guidelines for FTIR spectroscopy studies of biological samples. This will help new users to prepare samples for FTIR analysis and understand the critical steps to correctly perform spectra pretreatment, preprocessing and statistical analysis and to implement appropriate and evidence-based experimental designs.

Keywords:

• FTIR spectroscopy
• biological samples
• spectra acquisition
• spectral analysis of biological samples

Additional information

Funding

This work was developed within the scope of Institute for Biomedicine – iBiMED (UIDB/04501/2020 and POCI-01-0145-FEDER-007628), financed by the Portuguese Foundation for Science and Technology (FCT), Compete2020 and FEDER and the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through FCT/MCTES. Sandra Magalhães is also funded with an individual grant of FCT (SFRH/BD/131820/2017).