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Genetics and Resistance / Génétique et Résistance

Fungal development and host response between ‘Avocet S’ and ‘Avocet S +Yr10’ wheat challenged with stripe rust

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Accepted 31 May 2024, Published online: 21 Jun 2024
 

Abstract

Wheat stripe rust disease caused by Puccinia striiformis f. sp. tritici (Pst) seriously affects wheat yield. We studied the fungal infection process and host response between wheat ‘AvS’ and its near-isogenic line ‘AvS+Yr10’, which formed compatible and incompatible interactions, respectively, when challenged with Pst race CYR32. The difference in reactive oxygen species (ROS) accumulation between the compatible and incompatible interactions was analyzed by measuring the hydrogen peroxide (H2O2) concentration and observing H2O2 production. Hydrogen peroxide concentrations, H2O2 accumulation areas, necrotic areas and expression of several resistance-related genes in the AvS+Yr10/CYR32 interaction were higher than those in the AvS/CYR32 interaction during the entire infection process. Pst growth was effectively inhibited in the AvS+Yr10 all-stage resistant wheat line. Thus, ROS accumulation is crucial in wheat defence against pathogens.

Résumé

La maladie de la rouille jaune du blé causée par Puccinia striiformis f. sp. tritici (Pst) affecte sérieusement le rendement du blé. Nous avons étudié le processus d’infection fongique et la réponse de l’hôte entre le blé ‘AvS’ et sa lignée quasi-isogénique ‘AvS+Yr10’, qui ont formé des interactions compatibles et incompatibles, respectivement, lorsqu’ils ont été confrontés à la race CYR32 du Pst. La différence d’accumulation d’espèces réactives de l’oxygène (ROS) entre les interactions compatibles et incompatibles a été analysée en mesurant la concentration de peroxyde d’hydrogène (H2O2) et en observant la production de H2O2. Les concentrations de peroxyde d’hydrogène, les zones d’accumulation de H2O2, les zones nécrotiques et l’expression de plusieurs gènes liés à la résistance dans l’interaction AvS+Yr10/CYR32 étaient plus élevées que dans l’interaction AvS/CYR32 pendant toute la durée du processus d’infection. La croissance du Pst a été efficacement inhibée dans la lignée de blé résistante à tous les stades AvS+Yr10. Ainsi, l’accumulation de ROS est cruciale dans la défense du blé contre les pathogènes.

Acknowledgments

We would like to thank State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, China for the equipment support.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was supported by the National Natural Science Foundation of China [No.32172424] and 2022 College Students’ innovation and entrepreneurship training program [S202210712299].

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