Multidimensional Studies of Pancratium parvum Dalzell Against Acetylcholinesterase: A Potential Enzyme for Alzheimer’s Management

Abstract

Objective: Pancratium L. (Amaryllidaceae J.St. Hil.) is a monocot genus with bulbous habitat and about 20 species worldwide have significant medicinal properties. The present envision aims to investigate the potential ability of Pancratium species for acetylcholinesterase (AChE) inhibition as a remedy for Alzheimer disease (AD). Different Pancratium species were screened for the inhibition of AChE enzyme from various localities across India. Prominent species was further studied for anti-inflammatory, antioxidant, metal chelating and UHPLC-QTOF-MS analysis.

Methods: Nine different species collected across India were examined for AChE inhibition and for binding affinity studies using Surface Plasmon Resonance (SPR). Highest inhibition species was subjected to Response Surface Methodology (RSM) to accomplish the effective conditions for maximum extraction of phytomolecules in accordance with the inhibition of the AChE. Further, extract under optimized conditions were used to study anti-inflammatory, antioxidant, metal chelating and UHPLC-QTOF-MS analysis for tentative identification of phytomolecules.

Results: Amongst different species collected, P. parvum Dalzell exhibited maximum inhibition 93.30 ± 1.71% with promising IC₅₀ 20 ± 0.22 µg/ml value. In addition, binding affinity toward AChE and β plaques using SPR technique showed a higher binding response toward the enzyme. RSM study resulted that water extracts at 50 °C and 5.46 hours heating executed maximum inhibition. Other studies showed prominent anti-inflammatory and metal chelating ability with low antioxidant property.

Conclusion: By using UHPLC-QTOF-MS compounds were tentatively identified for the concerned activities mentioned above. This work reports for accounting the detailed study of P. parvum and which can be further entailed for the treatment of various neurological disorders.

Keywords:

• P. parvum
• inhibitory activity
• SPR
• RSM
• LCMS-QTOFMS
• anti-Alzheimer’s drug

Acknowledgments

DST-INSPIRE meritorious fellowship to Ms. D. N. Patil from DST, New Delhi, India is gratefully acknowledged. One of the author Dr. V. A. Bapat wish to thanks National Academy of Sciences, India for honorary Scientist Fellowship and Dr. S. R. Yadav to UGC for the award of faculty fellowship. Corresponding author also wish to thank Golden Jubilee funding from Government of Maharashtra for Instrumentation facilities like SPR and LC-MS QTOF. All authors wish to thank Dr. Onkar Apine for his significant contribution in designing an execution of LC QTOF MS experiments.

Disclosure statement

Authors declare that they have no conflict of interests.