Application of Ionic Liquid Modified Silica for Solid-Phase Extraction of Polysaccharides from Laminaria japonica

Abstract

Silica-confined ionic liquids were synthesized for solid-phase extraction of polysaccharides, which was determined by high-performance liquid chromatography coupled with RI detection. The sorbent with amino-imidazolium groups was found to be the optimal material by a comparison of the adsorption capacity of fucoidan and laminarin onto different synthetic sorbents. The proper elution solvents for both laminarin and fucoidan were decided from practical tests. The sorbent performed stably and selectively, demonstrating potential applications in the separation of hydrophilic biomacromolecules, such as polysaccharides.

Keywords:

• Solid-phase extraction
• Ionic liquid modified sorbents
• Laminarin
• Fucoidan