Abstract
A new oligosaccharide citric acid derivative was isolated by hot aqueous extraction at 85 °C from Vigna angularis seeds, and it was purified using anion exchange chromatography DE-52. The structure was inferred by using UV, 1 D and 2 D NMR spectroscopy and HR-MALDI/MS, and identified as α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyl-(1→6)-α-D-glucopyranosyle-(1→2)-β-D-fructofuranosyle citric acid. The isolated oligosaccharide citric acid derivative revealed promising anti-influenza A virus with 50% inhibition concentration (IC50) of 0.162 nM/mL and 50% cytotoxic dose (CC50) of >2.38 nM/mL.
Acknowledgements
The authors are grateful to the National Research Center, Cairo, Egypt, for funding the experimental work, including specific optical rotation, TOCSY and HMBC analysis [Project (No. AR110210)], and to the Nagasaki University for providing facilities for instrumental analysis.
Conflict of Interest
The authors declare that they have no conflict of interest.