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Nucleosides and Nucleotides Volume 5, 1986 - Issue 5
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Original Articles

Restricted Joining of a Decadeoxyribonucleotide for Preparation of an Eicosadeoxyribonucleotide Duplex Containing Recognition Sites for Restriction Enzymes

Y. Ishino Faculty of Pharmaceutical Sciences Osaka University, Osaka, Japan
,
S. Shibahara Faculty of Pharmaceutical Sciences Osaka University, Osaka, Japan
,
E. Ohtsuka Faculty of Pharmaceutical Sciences Osaka University, Osaka, Japan
&
M. Ikehara Faculty of Pharmaceutical Sciences Osaka University, Osaka, Japan
Pages 471-480 | Published online: 06 Dec 2006
 
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Abstract

A partially self-complementally decadeoxyribonucleotide, d(pCGACCCGGGT) has been 3′-blocked with the acetyl group and joined to d(CGACCCGGGT) to yield an eicosadeoxyribonucleotide duplex which contained recognition sites for six restriction enzymes.

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