Abstract
N4 aralkyl-substituted cytosine nucleosides, available dxectly by displacement of the PfpO group at C4 of 5′-O-DMT-protected nucleoside 4, were efficiently incorporated into short oligonucleotides. Aralkyl substitution at the N4 of cytosine was entropically stabilising but offset by loss in enthalpy resulting overall in duplex destabilisation.