Introduction of 6-Formylcytidine into a Myb Binding Sequence

Atushi Kittaka School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan

Tesuya Kuze School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan

Miori Amano School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan

Hiomichi Tanaka School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan

Taashi Miyasaka School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan

Kuihiko Hirose Toagosei Co., Ltd., 2 Ohkubo, Tsukuba, Ibaraki, 300-2611, Japan

Taao Yoshida Toagosei Co., Ltd., 2 Ohkubo, Tsukuba, Ibaraki, 300-2611, Japan

Aknori Sarai Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Tsukuba, Ibaraki, 305-0074, Japan

Taashi Yasukawa Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Tsukuba, Ibaraki, 305-0074, Japan

Shnsuke Ishii Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Tsukuba, Ibaraki, 305-0074, Japan

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Abstract

Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3-TTGAC-5′. The modified duplex showed Tm of 67°C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.

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Introduction of 6-Formylcytidine into a Myb Binding Sequence

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Introduction of 6-Formylcytidine into a Myb Binding Sequence

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