Combinatorial Library of Artificial Ribonucleases

Abstract

Conjugates of lanthanide complexes and oligonucleotides have been shown to bind and to cleave complementary RNA. Modified oligonucleotide building blocks are required to ensure stability of the oligonucleotide against cellular nucleases. For an efficient cleavage, an accessible site on the structured mRNA is required. For the identification of active oligoribonucleotide-lanthanide complex conjugates, we have used a combinatorial library of conjugate sequences.