Molecular Analysis of In Vitro Shoot Organogenesis

Abstract

Shoot organogenesis is one of the in vitro plant regeneration pathways. It has been widely employed in plant biotechnology for in vitro micropropagation and genetic transformation, as well as in study of plant development. Morphological and physiological aspects of in vitro shoot organogenesis have already been extensively studied in plant tissue culture for more than 50 years. Within the last ten years, given the research progress in plant genetics and molecular biology, our understanding of in vivo plant shoot meristem development, plant cell cycle, and cytokinin signal transduction has advanced significantly. These research advances have provided useful molecular tools and resources for the recent studies on the genetic and molecular aspects of in vitro shoot organogenesis. A few key molecular markers, genes, and probable pathways have been identified from these studies that are shown to be critically involved in in vitro shoot organogenesis. Furthermore, these studies have also indicated that in vitro shoot organogenesis, just as in in vivo shoot development, is a complex, well-coordinated developmental process, and induction of a single molecular event may not be sufficient to induce the occurrence of the entire process. Further study is needed to identify the early molecular event(s) that triggers dedifferentiation of somatic cells and serves as the developmental switch for de novo shoot development.

Keywords:

• shoot organogenesis
• in vitro
• shoot meristem
• cell cycle
• cyclin-dependent kinase
• cyclin
• cytokinin

ACKNOWLEDGEMENTS

We are very grateful to Drs. Michael Christianson and Malgorzata Gaj for their critical review of this manuscript, and to Barbara Alonso for help in the preparation of the figures and the references. We attempted to cite all pertinent references but apologize to any colleagues whose work was inadvertently missed.