Impaired Lymphocyte Responses and Their Restoration in Oral Cancer Patients Expressing Distinct TCR Variable Region

Abstract

We have analyzed TCR Vβ gene usage and clonality of T cells in the peripheral blood of patients with oral cancer and healthy individuals. A large repertoire of clonal TCR Vβ was observed in the peripheral blood of cancer patients, and this clonal expansion was dominantly represented in the CD8+ T cells. A marked decrease in the lymphocyte proliferative responses to mitogen and anti-CD3 MAb and spontaneous apoptosis was observed in lymphocytes. Appropriate co-stimulation of lymphocytes (anti-TCR Vβ MAb and anti-CD28 MAb) restored the lymphocyte proliferative responses and CD3-ζ chain expression in these patients.

Keywords:

• Oral cancer
• TCR Vβ clonality
• Lymphocyte proliferation
• Immune dysfunction
• Apoptosis
• CD3-ζ chain