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Cancer Investigation Volume 27, 2009 - Issue 5
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ORIGINAL ARTICLES Clinical Translational Therapeutics

The Effect of TGF-β2 on MMP-2 Production and Activity in Highly Metastatic Human Bladder Carcinoma Cell Line 5637

Ehsan Dehnavi Ministry of Science, Research and Technology, National Institute of Genetic Engineering and Biotechnology; Khatam University
,
Zahra-Soheila Soheili Ministry of Science, Research and Technology, National Institute of Genetic Engineering and Biotechnology
,
Shahram Samiei Iranian Blood Transfusion Organization Research Center
,
Zahra Ataei Iranian Blood Transfusion Organization Research Center
&
Hajar Aryan Payame-noor University of Tehran
Pages 568-574 | Published online: 20 Jul 2009
 
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Abstract

Transforming growth factor–beta (TGF-β) superfamily regulates matrix metalloproteinases (MMP), which intrinsically regulate various cell behaviors leading to metastasis. We investigated the effect of TGF-β2 on MMP-2 regulation in human bladder carcinoma cell line 5637. Zymography, ELISA, and real-time polymerase chain reaction revealed that TGF-β2 stimulated MMP-2 production, but the transcription of its gene remained unchanged. Wortmannin could not inhibit MMP-2 secretion and activity and conversely the amount of the protein and its enzymatic activity were increased. These data suggest that TGF-β2 increased MMP-2 at the posttranscriptional level and this upregulation was independent of phosphatidylinositol 3-kinase signaling pathway.

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