Expression of WISP3 and RhoC Genes at mRNA and Protein Levels in Inflammatory and Noninflammatory Breast Cancer in Tunisian Patients

ABSTRACT

Previous studies have shown the expression WISP3 and RhoC in cell lines of inflammatory breast cancer (IBC). The aim in the current study was to compare the expression of both genes, in biopsy samples collected from Tunisian patients with localized or metastatic breast cancer and patients with IBC.

We investigated 127 patients enrolled in Salah Azaiez Institute in Tunis. Using the RT-PCR, we showed the phenotype (WISP3−, RhoC+) is significantly associated with IBC tumors, while the (WISP3+, RhoC−)phenotype is mostly associated to non-IBC tumors. The frequencies of these tumor phenotypes are significantly different between these tumor groups (p = 10^{− 7}; relative risk or RR = 3.25; confidential interval or CI 95% = 1.90–5.53).

Immunohistochemical test revealing the presence of WISP3 and RhoC proteins correlates with the expression in the biopsy of their encoding genes as detected by RT-PCR.

In conclusion, it appears that WISP3 and RhoC genes expression status defines a molecular signature of IBC.

Keywords: :

• RT-PCR
• WISP3
• RhoC
• Immunohistochemistry
• Inflammatory breast cancer
• Tunisian population