Expression of Circadian Clock Genes in Human Colorectal Cancer Tissues Using Droplet Digital PCR

Abstract

Increasing evidence indicates that disruption of circadian rhythms may be directly linked to cancer. Here we report that the expression levels of the core clock genes Per1 and Per3 measured by droplet digital polymerase chain reaction are significantly decreased in tumour tissue from 16 patients undergoing colorectal cancer surgery compared to paired normal mucosa. No differences were observed in the expression of Per2, Bmal1, and Clock. In conclusion, abnormal expression levels of the clock genes Per1 and Per3 in CRC tissue may be related to tumourigenesis and may provide future diagnostic and prognostic information.

Keywords:

• Colorectal cancer
• Circadian clock genes
• mRNA
• ddPCR

Declaration of interest

The authors report no conflicts of interest.

Additional information

Funding

The study was supported by Spogaards Family Fund.