Elucidation of transfer RNAs as stress regulating agents and the experimental strategies to conceive the functional role of tRNA-derived fragments in plants

Abstract

In plants, the transfer RNAs (tRNAs) exhibit their profound influence in orchestrating diverse physiological activities like cell growth, development, and response to several surrounding stimuli. The tRNAs, which were known to restrict their function solely in deciphering the codons, are now emerging as frontline defenders in stress biology. The plants that are constantly confronted with a huge panoply of stresses rely on tRNA-mediated stress regulation by altering the tRNA abundance, curbing the transport of tRNAs, fragmenting the mature tRNAs during stress. Among them, the studies on the generation of transfer RNA-derived fragments (tRFs) and their biological implication in stress response have attained huge interest. In plants, the tRFs hold stable expression patterns and regulate biological functions under diverse environmental conditions. In this review, we discuss the fate of plant tRNAs upon stress and thereafter how the tRFs are metamorphosed into sharp ammunition to wrestle with stress. We also address the various methods developed to date for uncovering the role of tRFs and their function in plants.

Keywords:

• Plant-tRNAs
• animal-tRNAs
• stress-response
• tRNA-abundance
• tRNA-modification
• tRNA-fragmentation
• tRFs

Acknowledgements

A.U. acknowledges Department of Science and Technology (DST) – JRF & SRF. P.V. K.D. and E.V.S. greatly acknowledge the Department of Biotechnology (DBT).

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

This work was supported by the Rajiv Gandhi Center for Biotechnology and Department of Science and Technology under DST/INSPIRE Fellowship (DST/INSPIRE FELLOWSHIP 2015/IF150352).