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Journal of Biomolecular Structure and Dynamics Volume 7, 1990 - Issue 6
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Original Articles

Translation-Initiation Promoting Site on Transcripts of Highly Expressed Genes From Saccharomyces cerevisiae and the Role of Hairpin Stems to Position the Site Near the Initiation Codon

T. A. Thanaraj Centre for Cellular and Molecular Biology, Hyderabad, 500 007, India
&
M. W. Pandit Centre for Cellular and Molecular Biology, Hyderabad, 500 007, India
Pages 1279-1289 | Received 23 Mar 1990, Published online: 21 May 2012
 
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Abstract

It is reported that the AUG-upstream region on mRNAs of highly expressed genes from S. cerevisiae invariably possesses a translation-initiation promoting site, that can base pair with a well-conserved site on 18S rRNA during the formation of 40S initiation complex. Weak hairpin stem in the mRNA region between such a site and the initiation codon brings the site nearer to the initiation codon and also extends the length of base pairing. Such a base pairing can lead to a comparatively more stable 40S initiation complex, resulting in a higher rate of formation of the 80S initiation complex and consequently in an enhancement of the rate of initiation of translation. The site on 18S rRNA can interchange its base pairing between the site on mRNA and a well-conserved site on 25S rRNA in the formation of the 80S initiation complex.

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