Atomic Force Microscopy Of Uncoated Plasmid DNA: Nanometer Resolution with only Nanogram Amounts of Sample

Min-Qian Li Shanghai Institute of Nuclear Research Academia Sinica, Shanghai, 201800, China

Helen G. Hansma Department of Physics, University of California, Santa Barbara, CA, 93106, USA

James Vesenka Department of Zoology and Genetics, Iowa State University, Ames, IA, 50011

Gregory Kelderman Department of Physics, University of California, Santa Barbara, CA, 93106, USA

Paul K. Hansma Department of Physics, University of California, Santa Barbara, CA, 93106, USA

Abstract

Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 °C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA.

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Atomic Force Microscopy Of Uncoated Plasmid DNA: Nanometer Resolution with only Nanogram Amounts of Sample

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Atomic Force Microscopy Of Uncoated Plasmid DNA: Nanometer Resolution with only Nanogram Amounts of Sample

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