Structural Study of Homeodomain Protein-DNA Complexes Using a Homology Modeling Approach

Abstract

The homeodomain is a conserved protein motif that binds to DNA and plays a central role in gene regulation. We use homeodomain as a model system to study the specific interactions between protein and DNA in a complex. Following the fundamental concept of homology modeling, we have developed an algorithm for predicting structures of both protein and DNA using the known structure of a similar complex as the template. The accuracies of the algorithm in predicting the complex structures are evaluated when two of the homeodomain protein-DNA complexes with known structures (antennapedia and MAT α2) are selected as test systems. This algorithm allows structural studies of homeodomain binds to DNA with different sequences.