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Journal of Biomolecular Structure and Dynamics Volume 28, 2011 - Issue 6

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Original Articles

Structure and Stability Analysis of Cytotoxic Complex of Camel α-Lactalbumin and Unsaturated Fatty Acids Produced at High Temperature

Maliheh S. Atri Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Ali A. Saboury Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Ali A. Moosavi-Movahedi Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Bahram Goliaei Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Yahya Sefidbakht Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Hamid Hadi Alijanvand Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Ahmad Sharifzadeh Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Amir Niasari-Naslaji Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

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Pages 919-928 | Received 24 May 2010, Published online: 21 May 2012

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https://doi.org/10.1080/07391102.2011.10508618

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Abstract

α-Lactalbumin (α-La), together with oleic acid can be converted to a complex, which kills tumor cells selectively. Cytotoxic α-La -oleic acid and a-La -linoleic acid complexes were generated by adding fatty acid to camel holo a-La at 60°C (referred to as La-OA-60 and La-LA-60 state, respectively). Structural properties of these complexes were studied and compared to the camel α-La. The experimental results show that linoleic acid induces a-La partial unfolding but oleic acid does not change the protein structure significantly. Also the stability of La-OA-60 and La- LA-60 toward thermal denaturation was measured. The order of temperature at the transition midpoint is as follows: La-LA-60 < La-0A-60 < α-La. La-0A-60 complex inhibited tubulin polymerization in vitro. Although the structures of La-0A-60 and La-LA-60 were different, these two complexes had similar cytotoxic effect to DU145 human prostate cancer cells. Samples of La-0A-60 that have been renatured after denaturation lost the specific biological activity toward tumor cells.

Key words:

α-lactalbumin
Fatty acid
Fluorescence spectroscopy

Abbreviations

α-La	=	alpha-lactalbumin
MTT	=	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
DMSO	=	dimethyl sulfoxide
FBS	=	fetal bovine serum
PBS	=	phosphate buffered saline
La-OA-60 and La-LA-60	=	complexes of camel α-lactalbumin with oleic acid and linoleic acid, respectively, prepared at 60°C as described in Materials and Methods
RMSD	=	root mean square deviation

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Structure and Stability Analysis of Cytotoxic Complex of Camel α-Lactalbumin and Unsaturated Fatty Acids Produced at High Temperature

Abstract

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References

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Structure and Stability Analysis of Cytotoxic Complex of Camel α-Lactalbumin and Unsaturated Fatty Acids Produced at High Temperature

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