Binding analysis of antioxidant polyphenols with PAMAM nanoparticles

Abstract

Dietary polyphenols are abundant micronutrients in our diet and paly major role in prevention of degenerative diseases. The binding efficacy of antioxidant polyphenols resveratrol, genistein, and curcumin with PAMAM-G3 and PAMAM-G4 nanoparticles was investigated in aqueous solution at physiological conditions, using multiple spectroscopic methods, TEM images, and docking studies. The polyphenol bindings are via hydrophilic, hydrophobic, and H-bonding contacts with resveratrol forming more stable conjugates. As PAMAM size increased the loading efficacy and the stability of polyphenol-polymer conjugates were increased. Polyphenol binding induced major alterations of dendrimer morphology. PAMAM nanoparticles are capable of delivery of polyphenols in vitro.

Keywords:

• polyphenols
• dendrimers
• conjugation
• loading efficacy
• TEM
• modeling

Abbreviations:

• res, resveratrol
• gen, genistein
• cur, crucumin
• PAMAM, polyamidoamine
• LE, loading efficacy
• FTIR, Fourier transform infrared
• TEM, Transmission electron microscopy

Acknowledgments

The financial support of the Natural Sciences and Engineering Research Council of Canada (NSERC) to H.A. Tajmir-Riahi is highly appreciated.