In silico determination of transposon-derived miRNAs and targets in Aegilops species

Abstract

Transposable elements (TEs) are found almost in all living organism, shaping organisms’ genomes. miRNAs are noncoding RNA types which are especially important in gene expression regulations. Many previously determined plant miRNAs are identical/homologous to transposons (TE-MIR). The aim of this study was computational characterization of novel TE-related miRNAs and their targets in Aegilops genome by using stringent criteria. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed by BLAST2GO. Seventeen novel TE-related miRNAs in Aegilops genome were identified for the first time. GO analyses indicated that 40 targets played different roles in biological processes, cellular components and molecular functions. Moreover, these genes were involved in 10 metabolic pathways such as purine metabolism, nitrogen metabolism, oxidative phosphorylation, etc. as a result of KEGG analyses. Identification of miRNAs and their targets are significant to understand miRNA-TEs relationships and even how TEs affect plant growth and development. Obtaining results of this study are expected to provide possible new insight into Aegilops and its related species, wheat, with respect to miRNAs evolution and domestication.

Communicated by Ramaswamy H. Sarma

Keywords:

• Transposable elements
• noncoding RNA
• gene ontology
• gene regulation

Acknowledgements

This research did not receive any specific funding. The author thanks the Amasya University Central Research Lab. (AUMAULAB) for their kind understanding of using their facilities.

Disclosure statement

No potential conflict of interest was reported by the author.