Tetrazolo[1,5-a]quinoline moiety-based Os(IV) complexes: DNA binding/cleavage, bacteriostatic and photocytotoxicity assay

Abstract

Biological applications of platinum group metal-based complexes have been widely explored in synthetic and inorganic chemistry. The compounds have been subjected to DNA binding, DNA cleavage, In-vivo and In-vitro photocytotoxicity (HCT-116 cell line) and bacteriostatic activities. Binding constant of complexes are 1.42–5.62 × 10⁴ M⁻¹, whereas that of ligands are 1.12–4.72 × 10⁴ M⁻¹. K_sv of complexes are about 1.32–5.21 × 10³ M⁻¹, whereas K_f is about 1.24–6.83 × 10³ M⁻¹. IC₅₀ of compounds screened using HCT-116 cell line in dark are found to be 121–342 μg/mL. Whereas photocytotoxicity is found in the range of 48–316 μg/mL. Docking energy of molecules have been evaluated to evaluate efficacy of binding. Molecular docking energy of complexes are in the range of −286.00 to −303.11 kJ/mol. Whereas that of ligands are −254.03 to −282.96 kJ/mol. MIC of complexes are 47 ± 2.5 to 77.50 ± 7.5 μM. LC₅₀ values of ligands fall in the range of 4.05–19.72 μg/mL and that of Os(IV) complexes fall in the range of 3.99–15.99 μg/mL. The Os(IV) complexes dominate in proving its potentiality compared to N, N-donor ligands in biological activities.

Communicated by Ramaswamy H. Sarma

Keywords:

• DNA binding
• bacteriostatic
• osmium(IV)
• photocytotoxicity
• HCT-116

Acknowledgements

Authors are also thankful to UGC-CPEPA and UGC-CAS programmes running in institute.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors thankful to the Head, Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India, for providing the laboratory facilities, U. G. C., New Delhi for providing financial assistance of UGC BSR grant No. C/2015/BSR/Chemistry/1579.