Multispectroscopic analysis, atomic force microscopy, molecular docking and molecular dynamic simulation studies of the interaction between [SnMe₂Cl₂(Me₂phen)] complex and ct-DNA in the presence of glucose

Abstract

In this study, the spectroscopic methods (UV–vis, fluorimetric), Atomic force microscopy, and computational studies (molecular docking and molecular dynamic simulation) were used to investigate the interaction of [SnMe₂Cl₂(Me₂phen)] complex with CT-DNA in the presence of glucose. The results showed the complex in the medium containing glucose has less effect on calf thymus DNA (ct-DNA) than the medium without glucose. Cytotoxicity of [SnMe₂Cl₂(Me₂phen)] complex on MCF-7 cells was examined and showed Sn(IV) complex possesses potential cytotoxicity against this cell line. Molecular docking study showed that Sn(IV) complex interacts with DNA by groove binding mode. Radius of gyration (Rg) was smaller upon binding of the Sn(IV) complex suggesting a more compact structure of DNA in the presence of Sn(IV) complex.

Communicated by Ramaswamy H. Sarma

Keywords:

• Diabetes complication
• atomic force microscopy
• glucose
• Sn(IV) complex
• 56-dimethyl-110-phenanthroline
• DNA interaction
• molecular dynamic simulation

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

Financial support from Razi University Research Center is gratefully acknowledged.