Molecular docking and inhibition studies of vulpinic, carnosic and usnic acids on polyol pathway enzymes

Abstract

Aldose reductase (AR) and sorbitol dehydrogenase (SDH) are important enzymes of the polyol pathway. In the current study, inhibitory effects of vulpinic acid (VA) carnosic acid (CA) and usnic acid (UA) on purified AR and SDH enzymes were determined. These enzymes inhibition could be essential to prevent diabetic complications. AR and SDH enzymes were purified from sheep kidney. Then, VA, CA and UA were tested in various concentrations against these enzymes activity in vitro. K_I values were found to be as 1.46 ± 0.04, 5.13 ± 0.25 and 11.71 ± 0.27 μΜ for VA, CA and UA, respectively, for AR. K_I constants were found to be as 15.32 ± 0.34, 145.60 ± 2.17 and 213.40 ± 2.64 μΜ VA, CA and UA, respectively, for SDH. These findings indicate that VA, CA and UA could be useful in the treatment of diabetic complications.

Communicated by Ramaswamy H. Sarma

Keywords:

• Aldose reductase
• sorbitol dehydrogenase
• carnosic acid
• usnic acid
• vulpinic acid

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Research Fund of Ardahan University [grant number 2019-008], the Research Fund of Erzincan Binali Yıldırım University [grant number FBA-2017-501] and the Research Fund of Anadolu University [grant number 2102S003].