Synthesis, structural characterizations and in vitro cytotoxic activities of new sulfonamide-based Schiff base derivatives

Abstract

Synthesis of five different new compounds (1-5) were carried out. Their structures were characterized by spectroscopic methods such as Fourier transform infrared, Proton nuclear magnetic resonance, Carbon-13 nuclear magnetic resonance and Elemental analysis. Cytotoxic activities of five new sulfonamide based-Schiff base compounds were determined by MTT assay using A-2780 (human ovarian cancer) and MCF-7 (breast cancer) cell lines. LogIC₅₀ values of the sulfonamide derivates compounds were calculated by Graphpad Prism 12 programme after a 24-hour treatment for A2780 and MCF-7 cells. Comet assay experiments were performed to determine DNA damage using LogIC₅₀ concentrations of all compounds in A2780 and MCF-7 cells. All compounds significantly reduced A2780 and MCF-7 cell viability compared to control groups (p < 0.05). In addition, all compounds caused DNA damage in A2780 and MCF-7 cells (p < 0.05). These results show that the synthesized compounds exhibit cytotoxic effects against cancer cells and that the cause of cell death is due to DNA damage.

Communicated by Ramaswamy H. Sarma

Keywords:

• Sulfonamide derivative
• Schiff base
• spectroscopic
• cytotoxic activity
• cancer cell lines
• DNA damage

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

The author(s) reported there is no funding associated with the work featured in this article.