Nano-gel formulation of polyphenolic fraction of tobacco stem for wound healing and its inhibitory efficacies against the receptors of chronic wound development

Abstract

Current investigation deals with the gel formulation of purified polyphenol loaded nanoparticles for enhanced wound healing. Polyphenols purified using column chromatography an were characterized using HPLC in fractions. Silver nanoparticles were characterization using SEM, AFM, TEM, DLS. Cytotoxic effect was checked on L929 (Mouse fibroblast cells). Bioactive loaded nanoparticles were formulated in gel and subjected for physical characterization. Excision wound wistar rat models were established to measure the wound contraction measurement, histopathological studies, and biochemical assays. Docking and Simulation studies were performed to check the binding and stability with receptors progressing the chronic wound. From the column chromatography, B fraction has shown the presence of maximum polyphenolic content and as well as the polyphenol compounds such as quercetin, rutin and tannic acid as confirmed by the HPLC. Synthesize B fraction-AgNP were found to be below 100 nm in size and spherical in shape as per the characterization. No cytotoxic effect was observed on L929 cells treated with B fraction and its nanoparticles. Formulated B fraction-AgNP gel has revealed the better stability and earlier wound contraction, i.e. before the completion of 14 days of topical treatment. Histopathology studies and collagen content has confirmed the re-epithelization in skin wounds. MD simulation revealed the stability with PI3K and AKT. This can be concluded that tobacco stem polyphenol loaded nanoparticles has potent wound healing activity that can be utilized as nano-drug delivery system for suppression of chronic wound development.

Communicated by Ramaswamy H. Sarma

Keywords:

• Polyphenol
• tobacco
• nanogel
• wound healing
• collagen
• nanoparticle

Acknowledgments

We authors would like to acknowledge Prof. (Dr.) Satyendra Kumar Rajput, Head of Department, Department of Pharmaceutical Science, Gurukul Kangri Vishwavidyalaya, Uttarakhand, India and Mr. Sudhir Kumar Pal, Assistant Professor, IILM University, Greater Noida, India for their valuable guidance and support.

Author contribution

Yash Sharma: Experimental, Analysis, Writing – Original draft preparation, Methodology, Investigation. Rohit Bhardwaj and Amritpal Kaur: Analysis, Investigation, Methodology and Reviewing. Gagandeep Singh: Analysis, Investigation, Writing, Methodology, Experimental analysis. Sharvari Kulkarni: Analysis, Investigation, Methodology, Experimental analysis. Amit Bhatia: Investigation, Experimental Analysis. Kumud Bala: Conceptualization, Methodology, Supervision.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Authors would like to thanks to Science and Engineering Research Board, Department of Science and Technology, India for providing fund for the project. The Grant number is EMR/2016/005824.