Publication Cover
Chronobiology International
The Journal of Biological and Medical Rhythm Research
Volume 36, 2019 - Issue 2
171
Views
9
CrossRef citations to date
0
Altmetric
Original Articles

Daily rhythms of leukocytes populations, biochemical and enzymatic blood parameters in a carnivorous freshwater catfish (Lophiosilurus alexandri)

, , , , , & show all
Pages 276-287 | Received 15 Jun 2018, Accepted 15 Oct 2018, Published online: 29 Oct 2018
 

ABSTRACT

The aim of this study was to investigate the daily rhythms of hematological, biochemical and enzymatic parameters of the blood of a nocturnal model of fish (Lophiosilurus alexandri) bred in the laboratory (F1). Thirty-six juveniles were stocked in six tanks of a recirculation aquaculture system for 20 days. The fish were exposed to a light:dark cycle of 12:12 h and were fed 1% of biomass twice a day with commercial diet. The daily rhythms of hematological, biochemical and enzymatic parameters were then measured at six sampling times “zeitgeber time = ZT” at four-hour intervals under light:dark 12:12 h (lights on = ZT0, at 8.00 a.m). No differences were observed to alkaline phosphatase, glucose, cortisol, aspartate aminotransferase, superoxide dismutase, total protein and hematocrit (p > 0.05). However, white blood cell count, Lymphocytes (LYN), Neutrophils (NEU), Eosinophil and Neutrophils to Lymphocytes ratio were significant different between sample times (p < 0.05). Also, a significant difference in alanine transaminase was observed, with a peak of production at nighttime. In contrast, glutathione peroxidase peaked at 8:00. Uric acid, magnesium and Calcium (Ca++) showed statistically significant differences (p < 0.05). A significant difference was observed (p < 0.05), with a peak of albumin at 08:00 and triglycerides at 12:00, while cholesterol was low (p < 0.05) at 08:00 and higher from 12:00 to 04:00. Cosinor analysis revealed also rhythmicity to SOD, UA, Mg and Ca++, ALB and CHO (p < 0.05). In conclusion, the time of day must be considered a key factor when using blood parameters as biomarkers for disease, health and welfare in the L. alexandri aquaculture.

Acknowledgments

We thank Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq-Brazil, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES-Brazil, and Fundação de Amparo a Pesquisa de Minas Gerais-FAPEMIG-Brazil for financial support and funding for the authors, including Ronald K. Luz (CNPq –Proc. 305048/2015-5) and Rodrigo Fortes (Produtividade em Produtividade-PQ CNPq –No.305734/2016-4 and 401416/2014-3).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico [305048/2015-5,305734/2016-4,401416/2014-3].

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 489.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.