ABSTRACT
Excessive sucrose intake, known as fructose toxicity, leads to fatty liver, hyperlipidemia, and metabolic syndrome. Circadian disorders also contribute to metabolic syndrome. Here, we investigated the effect of excessive sucrose intake on circadian rhythms of the small intestine, the main location of sucrose absorption, to elucidate a mechanism of sucrose-induced abnormal lipid metabolism. Male Wistar rats were fed control starch or high-sucrose diets for 4 weeks. High-sucrose diet-induced fatty liver and hypertriglyceridemia in rats. Amplitudes of PER1/2 expression oscillations in the small intestine were reduced by excessive sucrose, while gene expression of GLUT5 and gluconeogenic enzymes was enhanced. These changes would contribute to interfering in lipid homeostasis as well as adaptive responses to control fructose toxicity in rats.
Acknowledgments
We thank the Japan Society for the Promotion of Science (JSPS) for funding supports (Recipient: H.O.; No.21658052; No.25292069; No.16H04922). And S.S. is a recipient of Otsuka Toshimi Scholarship Foundation.
Author contributions
S.S. and H.O. conceived and designed the experiments; S.S. and F.H. performed the experiments; S.S., F.H., M.U., Y.M., S.M. and S.I. analyzed data; N.N., S.I., S.M. and H.O. contributed reagents/materials/analysis tools; S.S. and H.O. wrote the manuscript.
Declaration of Interest statement
The authors declare no competing interests.
Data availability
The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.
Supplementary Material
Supplemental data for this article can be accessed here.