Age-dependent altered redox homeostasis in the chronodisrupted rat model and moderation by melatonin administration

ABSTRACT

Circadian disruption or chronodisruption (CD) occurs when day-night cycles and other internal rhythms are not adjusted to environmental light-dark regimens and are unable to synchronize among each other. Artificial light-induced oxidative stress is a major concern as the circadian physiology of the cell is chronically altered due to suppression of the time-keeping hormone, melatonin. The relationship between age-related impaired redox status and disrupted circadian rhythms is still not fully understood. The present study evaluated the effect of artificial light at night (ALAN) with respect to aging and role of melatonin supplementation. This study was conducted on young (3 months) and old (24 months) male Wistar rats subdivided into four groups control (C), melatonin treated (MLT), artificial light at night (ALAN), and ALAN+MLT group. Pronounced changes were observed in the old compared to the young rats. Reactive oxygen species (ROS), malondialdehyde (MDA), plasma membrane redox system (PMRS), protein carbonyl (PCO), and sialic acid (SA) were significantly (p ≤ 0.05) increased, while ferric reducing ability of plasma (FRAP) and reduced glutathione (GSH) were significantly (p ≤ 0.05) suppressed in light-exposed young and old animals compared to their age-matched controls. Advanced oxidation protein products (AOPP) increased non-significantly in young rats of the ALAN group; however, significant (p ≤ 0.05) changes were observed in the old rats of the ALAN group compared to their respective controls. Advanced glycation end products (AGEs) increased and acetylcholinesterase (AChE) activity decreased, significantly (p ≤ 0.05) in young animals of the ALAN group, while nonsignificant changes of both parameters were recorded in the old animals of the ALAN groups compared with their age-matched controls. Melatonin supplementation resulted in maintenance of the normal redox homeostasis in both young and old animal groups. Our study suggests that aged rats are more susceptible to altered photoperiod as their circadian redox homeostasis is under stress subsequent to ALAN. Melatonin supplementation could be a promising means of alleviating age-related circadian disturbances, especially in light-polluted areas.

KEYWORDS:

• Aging
• artificial light-at-night
• circadian disruption
• melatonin
• oxidative stress

Declaration of interest statement

The authors declare no conflicts of interest.

Additional information

Funding

AKV would like to acknowledge Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing Junior Research Fellowship (Sanction letter No. 09/001(0426)/2018-EMR-I). The Department of Biochemistry is beneficiary of FIST grant of DST, New Delhi, and SAP DRS I from UGC, India.