Effects of 5-HT7 receptors on circadian rhythm of mice anesthetized with isoflurane

ABSTRACT

The role of the serotonin 7 receptor (5-HT₇ receptor) subtype in a number of domains has been widely recognized, but its role in the regulation of changes of the circadian rhythm after anesthesia is still unclear. We used intraperitoneal injection of 5-HT₇ receptor agonist LP-211 or antagonist SB-269970 in mice to influence the level of 5-HT₇ receptor protein in the SCN and to observe the role of this receptor on circadian rhythm changes after isoflurane anesthesia. Our results show the appropriate dose of SB-269970 significantly alleviated the circadian rhythm disorder induced by isoflurane anesthesia, while LP-211 significantly aggravated it after anesthesia, which is different from the phase shift that can be caused by the administration of LP-211 before anesthesia. These findings may indicate the 5-HT₇ receptor plays a complex role in the regulation of circadian rhythm after anesthesia. Our findings may provide some positive significance for alleviating circadian rhythm disorder in patients after anesthesia and ultimately promoting rapid postoperative recovery.

KEYWORDS:

• Anesthesia
• circadian rhythm
• serotonin 7 receptor
• phase shift
• suprachiasmatic nucleus
• mouse model

Declaration of interest

The authors declare no conflict of interest.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China (No.81701371,No.81730033), the Natural Science Foundation of Jiangsu Province (No.BK20170654), and the Key Talents of 13th Five-Year Plan for Strengthening Health of Jiangsu Province (No.ZDRCA2016069).