Effects of rhg-csf on neutrophil functions and survival in sepsis induced diabetic rats

Abstract

Diabetic patients are more prone to infection and evidence for an immunologic defect superimposed upon the metabolic abnormalities of diabetes is convincing. Neutrophils play a critical role in the host defense mechanism against various bacterial infections, and it is suggested that impaired neutrophil functions cause susceptibility to infections in diabetic patients. To explore the possibility that Granulocyte colony-stimulating factor (G-CSF) may be useful to prevent the morbidity and mortality caused by infections in diabetics. We studied the effect of G-CSF against septicemia in diabetic rats.

Forty eight rats were divided into seven equal groups. The II^nd. IV^th-VII^th groups were made diabetic by single intraperitoneal injection of streptozotocin. Fourth, Vtth and Vllth groups were made sepricemic by cecal ligation and perforation at the end of the second week of streptozotocin injection. G-CSF was subcutaneously injected into III^rd, V^th and VI^th groups. White blood cell count, neutrophil counts and function were determined. Rats in all groups were also observed for seven days for survival.