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Summary
Serotonin receptors were identified in Spisula sperm membranes by radioligand binding assay. Sperm membranes were prepared by nitrogen cavitation and by density gradient ultracentrifugation. Specific binding of [3H]5-HT to the receptors of isolated sperm membranes was determined. The dissociation constant was 2.7 nM and the maximum binding capacity was 11.25 pmoles/mg of protein. The order of decreasing potency of 5-HT agonists in blocking [3H]5-HT binding to the sperm membrane was as follows: 2-methyl 5-HT> 8-OH-DPAT> 5-HT> 5-CT> α-methyl-5-HT; and that of 5-HT antagonists was: ICS-205-930> BMY-7379> mianserin> methysergide. These findings suggest that the 5-HT receptors in Spisula sperm membranes contain sites that interact with 5-HT3 and 5-HT1 analogs.