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Abstract
We have investigated the efficacy of the combined use of Alum and inactive Bordetella pertussis (iBP) adjuvants for eliciting anti-peptide antibodies. ICR mice were immunized four times at 3-week intervals with each of 7 free (i.e., not conjugated to any carrier) synthetic peptides of 15–17 amino acid residues in Alum + iBP, in the commonly used adjuvant protocols (CFA; CFA (initial) followed by IFA), or in CFA + iBP. Serum samples after 3 and 4 injections were tested by RIA. Use of Alum + iBP greatly increased the production of antibodies for most of the peptides. The results have important implications for human vaccine formulation involving peptides.
| ABBREVIATIONS | ||
| Ab, Abs: | = | antibody |
| Ag: | = | antigen |
| Alum: | = | aluminum hydroxide |
| CFA: | = | complete Freund’s adjuvant |
| CPM: | = | counts per minute |
| hHb: | = | human hemoglobin |
| IFA: | = | incomplete Freund’s adjuvant |
| PBS: | = | 0.15M NaCl in 0.01M sodium phosphate buffer, pH 7.2 |
| iBP: | = | inactive organism of Bordetella pertussis |
| tAChR: | = | Torpedo californica acetylcholine receptor |