Interferon Regulatory Factor 5 Gene Polymorphisms in Iranian Women with Unexplained Recurrent Pregnancy Loss

ABSTRACT

Objective: This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran.

Methods: 176 women with unexplained RPL and 173 healthy postmenopausal controls were enrolled in this case-control study. Genotyping of the polymorphisms rs10954213 and rs3757385 was carried out using touchdown tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS PCR), and polymorphism rs41298401 was typed using PCR-restriction fragment length polymorphism (PCR-RFLP).

Results: Genotype frequencies were significantly different between RPL cases and controls regarding AG heterozygote genotype of rs10954213, GT genotype of rs3757385, and GG genotype of rs41298401. In addition, allele variants (G for rs10954213, T for rs3757385, and G for rs41298401) showed protective role against RPL, while GG haplotype of two first variants was shown to be a susceptibility factor for the disease.

Conclusion: These data provide the first evidence, to our knowledge, of the protective role of the studied IRF5 gene polymorphisms against unexplained RPL among Iranian women from south of Iran.

KEYWORDS:

• Haplotype analysis
• interferon regulatory factor 5
• polymorphism
• recurrent pregnancy loss

Acknowledgments

The authors thank Dr. Minoo Zolghadri (gynecologist) and her staffs for collaborating in sampling. We also thank Nosaybe Jaafari and Najmeh Noruzi for technical assistance. The data were provided from the thesis of the R. Amiri Jahromi, MSc student of molecular genetics in Islamic Azad University, Arsanjan Branch.

Declaration of interest

The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the paper.