ABSTRACT
Background: This study was conducted to compare the levels of maternal serum soluble endoglin (sEng) and cell-free fetal DNA (cffDNA) in pregnant females with PE to normotensive pregnant ones, together with relating these levels to preeclampsia (PE) severity and onset.
Method of the study: It was a comparative study in Mansoura University Hospital, Egypt, to detect the levels of serum sEng by ELISA besides the levels of cffDNA by quantitative real-time polymerase chain reaction in 80 pregnant females suffering from PE in addition to 80 normotensive pregnant ones that were included as control.
Results: Levels of serum sEng and cffDNA were higher in PE cases than control (p < 0.0001٭ both) and were significantly related to the severity of the disease. Levels were also higher in early than late onset PE (p < 0.003٭ and <0.002٭, respectively). Sensitivities, specificities, positive, and negative predictive values in addition to accuracy of serum sEng and cffDNA were 97.5%, 98.8%, 98.7%, 97.5%, and 98.1% and 97.5%, 93.8%, 94.0%, 97.4%, and 95.6%, respectively.
Conclusion: Maternal serum sEng and cffDNA can be good markers for diagnosis of PE in Egyptian patients. They are positively related to the disease severity.
Abbreviations: cffDNA; Cell-Free Fetal DNA, sEng; soluble Endoglin, PE; preeclampsia, qRT PCR; Quantitative real-time polymerase chain reaction.
Compliance with Ethical Standards
This research study fully complies with the ethical standards. Our study approval number was R.18.07.231.
Declarations of interest
The authors declare that they have no competing interests.
Informed consent
Informed consent was withdrawn from all individual participants included in the study.