No Association Between Interleukin 6 and Inducible Nitric Oxide Synthase Polymorphisms and Dengue Infection: A Case-Control Study

ABSTRACT

Background: Dengue is a life-threatening disease. The factors that lead to severe cases are not completely understood. The host immune system is involved in the response to infections and plays an important role in dengue infection. IL-6 and iNOS are components of the immune system and genetic polymorphisms in these genes may be involved in dengue virus infection. The study aimed to investigate the association of genetic polymorphisms in the IL6 and iNOS genes and dengue. Methods: We performed a case-control study using 60 dengue-infected individuals and 119 healthy controls. Polymorphisms in the IL6 (T15A) and iNOS (−1173CT) genes were amplified by Real-Time PCR. Statistical analyses were performed using BioEstat 5.0. Results: We identified that the frequency of T/A genotype of IL6 was higher in dengue fever patients and C/T genotype of iNOS was higher in dengue hemorrhagic fever patients, however, no association was found between these polymorphisms and dengue. Conclusion: Polymorphisms in iNOS and IL6 were not associated with dengue infection.

KEYWORDS:

• DENV
• genetic variation
• intercellular-signaling protein
• nitric oxide synthase type II

Disclosure statement

No potential conflict of interest was reported by the author(s).

Authors contribution

BRCS: Conceptualization, Formal analysis, Investigation, Data curation, Visualization, Writing – Original draft. ACMS: Formal analysis, Investigation, Writing- review & editing. DMB: Investigation. LDSS: Investigation. FASP: Investigation. ISBT: Writing- review & editing. KFF: Writing- review & editing. EVMSF: Conceptualization, Validation, Supervision, Writing – review & editing.

Additional information

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.