Nitric Oxide Synthase Gene Polymorphisms in Children with Primary Nocturnal Enuresis: A Preliminary Study
Sirs,
We appreciate the opportunity to respond to the thoughtful and correct comments of Dr. Heitor Moreno, Jr., regarding our article “Nitric Oxide Synthase Gene Polymorphisms in Children with Primary Nocturnal Enuresis: A Preliminary Study.”Citation[1] As he suggests, haplotype analysis is a more powerful approach in genetic studies than the analysis of single polymorphisms.Citation[2]
We re-eveluated our data and compared the distribution of estimated haplotypes frequencies in enuretics and controls by using SNPStats.Citation[3] However, we found no significant differences in the overall distribution of eNOS haplotype frequencies between groups with the present data. As was mentioned in the article, this was a preliminary study, and we continue the same study by increasing the number of subjects. We agree with Dr. Moreno Jr.'s comment that significant differencies in the distribution of eNOS haplotypes frequencies may be found in enuretic children when compared with healthy subjects.
We hope to share our observations with you in the near future.
REFERENCES
- Balat A, Alasehirli B, Oguzkan S, Gungor M. Nitric oxide synthase gene polymorphisms in children with primary nocturnal enuresis: A preliminary study. Renal Failure. 2007; 29: 79–83
- Tanus-Santos JE, Casella-Filho A. Endothelial nitric oxide synthase polymorphisms and susceptibility to hypertension: genotype versus haplotype analysis. Hypertension. 2007; 49: E1
- Sole X, Guino E, Vals J, Iniesta R, Moreno V. SNPStats: A Web tool for the analysis of association studies. Bioinformatics. 2006; 22: 1928–1929, DOI:10.1093/Bioinformatics/btl268.