Extracellular Production of a Novel Ice Structuring Protein by Bacillus subtilis—a Case of Recombinant Food Peptide Additive Production

Abstract

Bacillus subtilis is an ideal host for the production of extracellular heterologous proteins. Antifreeze protein (ice structuring protein, ISP) is a potent additive in cryogenic preservation of food products. In this investigation, the first production of a recombinant ISP was achieved by using a high-efficient artificial promoter and a signal peptide from subtilisin YaB. The functional ISP was expressed extracellularly at a high level by a protease deficient host, B. subtilis WB800. This study provides an efficient tool to produce recombinant food peptide additive at a high level and overcomes the problem of natural resources limitation as well.

Key Words:

• ice structuring protein
• extracellular production
• Bacillus subtilis

ACKNOWLEDGEMENTS

This work was supported by a grant (NSC 90-2313-B-005-160) from the National Science Council (NSC), Taiwan, Republic of China.

Notes

Yeh, C.M., Wang, J.P. (2006b). Antifreeze protein analogue and nucleic acid coding sequence thereof, and production and use of the same. Asia Pacific Intellectual Property Association. Applying No.95118228.