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Food Biotechnology Volume 21, 2007 - Issue 2
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Original Articles

Enhancement of Recombinant Subtilisin YaB Production by Bacillus subtilis

Chuan-Mei Yeh Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, Republic of ChinaCorrespondence[email protected]
,
Fang-Shang Su Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, Republic of China
,
Yu-Yi Liao Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, Republic of China
&
Ying-Chieh Tsai Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, Republic of China
Pages 105-117 | Published online: 22 Jun 2007
 
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Abstract

Bacillus subtilis is an ideal host for the production of extracellular heterologous proteins. The use of a strong, constitutive promoter is a good means of optimizing the expression of heterologous proteins. In this study, high level extracellular production of subtilisin YaB, a potent meat tenderizer, was achieved by engineering the wild-type subtilisin YaB promoter into an artificial promoter and expressed in Bacillus subtilis host. This artificial promoter provides an efficient tool to produce mutant subtilisin YaB or other recombinant heterologous proteins by B. subtilis at high level.

ACKNOWLEDGEMENTS

This work was supported by a grant (NSC 90–2313-B-005–160) from the National Science Council (NSC), Taiwan, Republic of China.

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