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Food Biotechnology Volume 22, 2008 - Issue 4
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Original Articles

Establishment and Application of PCR-SSCP Profile for Molecular Typing of Cordyceps Fungi

Hsiao-Che Kuo Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, Taiwan
,
Yong-Lin Su Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan
,
Huey-Lang Yang Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Research Center of Ocean Environment and Technology, National Cheng Kung University, Tainan, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, Taiwan
&
Tzong-Yueh Chen Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Research Center of Ocean Environment and Technology, National Cheng Kung University, Tainan, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, TaiwanCorrespondence[email protected]
Pages 311-325 | Published online: 06 Nov 2008
 
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Abstract

A method for distinguishing isolates of the fungi belonging to genus Cordyceps, used as food/medicine in Eastern Asia, was developed. A PCR-single stranded conformation polymorphism-based profile was applied and established on the ITS2 region of rDNA for molecular typing in 48 different isolates of Cordyceps species. Compared with the DNA sequencing data and secondary structure prediction of single strand DNA, the method used was sensitive and could distinguish between two closely related species in the genus Cordyceps. This method could be very useful for confirming the identity of a specific strain of Cordyceps for researchers and food companies that produce the fermented Cordyceps products as a quality control method.

ACKNOWLEDGMENTS

We thank Dr. Graham D. Wright for his suggestion on this manuscript. We also express our gratitude to the Ministry of Education, Taiwan, for supporting this research.

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