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Abstract
Objective: The cross-presentation of cell-associated autoantigens contributes to systemic autoimmune diseases, including systemic lupus erythematosus (SLE). Little is known about the regulation of the immune response against soluble autoantigens targeted in these diseases.
Methods: We immunized the offspring of New Zealand Black and New Zealand White mice (NZB × NZW F1) with syngeneic dendritic cells (DC) that had macropinocytosed β2-glycoprotein 1 (β2GPI) during propagation in normal mouse serum or that had phagocytosed apoptotic thymocytes with syngeneic (murine) or xenogeneic (bovine) β2GPI, which was associated to plasma membrane of the cells. Mice were in parallel immunized with apoptotic thymocytes that had associated the β2GPI to their membranes in the absence of DC. The development of anti-β2GPI antibodies and clinical features were monitored.
Results. Apoptotic cells alone, opsonized with β2GPI, failed to induce anti-β2GPI autoantibodies or clinical disease. In contrast, autoimmunity developed in the presence of DC. Furthermore, the syngeneic β2GPI was a more effective antigen than the xenogeneic protein in re-boosted animals.
Conclusions. DC effectively initiate in NZB × NZW F1 mice self-sustaining autoimmunity against the β2GPI, either associated to apoptotic cells or macropinocytosed from the serum.
| Abbreviations | ||
| (SLE) | = | systemic lupus erythematosus |
| (DCs) | = | dendritic cells |
| (IFA) | = | incomplete Freund's adjuvant |
| (NZB × NZW F1) | = | offspring of New Zealand Black and New Zealand White mice |
| (AxV) | = | annexin V |
| (ANA) | = | anti-nuclear antibodies |
| (β2GPI) | = | β2 Glycoprotein I |
| (anti-β2GPI IgG) | = | anti-β2GPI antibodies |
| Abbreviations | ||
| (SLE) | = | systemic lupus erythematosus |
| (DCs) | = | dendritic cells |
| (IFA) | = | incomplete Freund's adjuvant |
| (NZB × NZW F1) | = | offspring of New Zealand Black and New Zealand White mice |
| (AxV) | = | annexin V |
| (ANA) | = | anti-nuclear antibodies |
| (β2GPI) | = | β2 Glycoprotein I |
| (anti-β2GPI IgG) | = | anti-β2GPI antibodies |
Notes
*Supported by the AIRC, by the E.C. (APOCLEAR), by the MIUR (to AAM and to MGS).