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Original Articles

Nanomedicine-mediated prevention of inflammatory monocytes infiltration ameliorate ovalbumin-induced allergic rhinitis in mouse model

, , , , , & show all
Pages 218-224 | Received 15 Dec 2019, Accepted 29 Mar 2020, Published online: 14 Apr 2020
 

Abstract

Th2 immune cells infiltration into nasal mucosa is one of the characters of allergic rhinitis (AR). We aimed to explore whether inhibition of Th2 immune cells infiltration would attenuate AR progression. AR mouse model was established by i.p. injection of ovalbumin (OVA). The infiltrated immune cells into nasal lavage fluid were detected by flow cytometry. Cytokine concentration in serum was determined by ELISA. AR mice symptoms were indicated by the number of sneezing and nasal rubbing events. In AR mice, CCL2 expression levels and CD45+CD11b+Ly6Chi inflammatory monocytes cells significantly increased as compared with control mice. CCL2 siRNA encapsulated nanoparticles (NPsiCCL2) prevent CCL2 expression and inflammatory monocytes infiltration in AR mice. NPsiCCL2 treatment dramatically decreased the number of sneezing and nasal rubbing events in AR mice. Moreover, NPsiCCL2 treatment attenuated serum OVA-specific IgE, OVA-specific IgG1 and histamine levels. Mechanically, NPsiCCL2 treatment attenuates AR symptoms via inhibiting Th2 cytokine (IL-4, IL-5 and IL-13) production. Nanomedicine-mediated prevention of inflammatory monocytes infiltration ameliorates ovalbumin-induced allergic rhinitis in mouse model.

Ethics approval

The mice were used under the guidelines of approved animal protocols by the committee of animal use and care in the First Affiliated Hospital, Nanjing Medical University.

Author’s contributions

XFG, FX, WML and YX were involved in study design, conducted the experiments, analyzed and interpreted data and was involved inwriting the manuscript. XL was involved in study design, analyzed and interpreted data and was involved in writing the manuscript. CJY and XYC were involved in the conceptualization of the study, study design provided funding, the analyzed and interpreted data and was involved in the writing of the manuscript. All authors have read and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reason-able request.

Additional information

Funding

This work was supported by grants from Nanjing Medical Science and Technique Development Foundation (QRX17051), the Project of Invigorating Health Care through Science, Technology and Education (ZDXKB2016015) and National Natural Science Foundation for Young Scientists of China (Grant No. 81600789).

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