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Research Articles

microRNA-130b-3p delivery by mesenchymal stem cells-derived exosomes confers protection on acute lung injury

, , , , , , & show all
Pages 597-607 | Received 01 Dec 2021, Accepted 18 Jun 2022, Published online: 26 Aug 2022
 

Abstract

Objective

Researchers have investigated miR-130b-3p in lung disease pathology, such as lung fibrosis. The present study was performed to elucidate the miR-130b-3p-involved mechanism in acute lung injury (ALI) through delivery by mesenchymal stem cells-derived exosomes (MSCs-Exo).

Methods

ALI mouse models were induced via intratracheal administration of lipopolysaccharide (LPS) and treated with MSCs-Exo. Lung dry-wet (W/D) ratio, inflammatory factors in the bronchoalveolar lavage fluid, pathological damage and apoptosis in the lung tissues were analyzed. Expression levels of miR-130b-3p and TGFBR1 were measured in the mouse lung tissues, and the interaction between miR-130b-3p and TGFBR1 was studied.

Results

MSCs-Exo relieved LPS-induced ALI in mice by reducing lung W/D ratio and inflammatory response, and attenuating lung tissue pathological damage and reducing the alveolar cell apoptosis. miR-130b-3p delivery by MSCs-Exo reduced LPS-induced ALI in mice. TGFBR1 was determined to be a downstream target gene of miR-130b-3p. Inhibition of TGFBR1 could remit LPS-induced ALI in mice. The protection mediated by MSCs-Exo carrying miR-130b-3p could be rescued by elevating TGFBR1 expression. 

Conclusion

miR-130b-3p delivery by MSCs-Exo confers protection on ALI in mice via the downregulation of TGFBR1.

Disclosure statement

The authors declare no competing interests.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (No. 81760020, No. 82060024), the Youth Project of Nursery Program 2019 in the Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region (No. GXWCH-YMJH-2018006), and Guangxi Anesthesiology Clinical Medicine Research Center Construction Project (scientific foundation of guangxi No: AD22035214).

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