Pretreatment of Mice with Rifampicin Prolongs Survival of Endotoxic Shock by Modulating the Levels of Inflammatory Cytokines

Abstract

To investigate the effects of rifampicin in a mouse model of endotoxin shock, mice were pretreated with rifampicin for at different times before and after challenging with a lethal dose of lipopolysaccharide (LPS). We found that rifampicin had a significant preventive effect and reduced the mortality of mice at early stages. To further understand the mechanism, plasma cytokine levels were examined. Mice treated with LPS alone showed markedly increased plasma levels of TNF, IL-1β, IL-6, and IL-10, while mice pretreated with rifampicin showed significantly lower plasma levels of these cytokines compared to the LPS alone. Our results suggest that rifampicin has a beneficial effect on septic shock caused by LPS through modulation of cytokines. This suggests that rifampicin could be a candidate for treatment of septicemia caused by gram-negative bacterial infection.

Keywords:

• Rifampicin
• LPS
• Mice
• Survival
• Cytokines

ACKNOWLEDGMENTS

This work was supported by The National Basic Research Program (973 programs) (2006CB504402).