Growth-inhibitory effects of a Bulnesia sarmienti aqueous extract on A549 Cells in vitro and S180 cells in vivo

Abstract

The effects of Bulnesia sarmienti (BS) aqueous extract on the cell growth of A549 cell lines were investigated. BS has strong cytotoxic activity on the A549 cell lines (IC₅₀; less than 100 μ g/mL) in MTT assay. HPLC confirmed that BS contains catechins as major compound. Cell cycle analysis by flow cytometry indicated that BS arrested the cell cycle in the sub-G₁ phase. BS induced DNA fragmentation, and increased the expression of the p53 protein in immunoblot analysis. These results indicated that the anticancer effect of BS was mediated via the process of apoptosis and growth-inhibition.

Keywords::

• Bulnesia sarmienti
• growth inhibition
• A549 cells
• cytotoxicity
• apoptosis

Acknowledgments

This work was supported by a grant (Technical Development Project No. 70000179) from Regional Industrial Promotion Projects by Ministry of Knowledge Economy, Republic of Korea.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.