MicroRNA-582-3p regulates osteoporosis through regulating homeobox A10 and osteoblast differentiation

Abstract

Objective

Recent studies have demonstrated that micro RNAs (miRNAs) are involved in bone formation and bone cell differentiation, but the role of miR-582-3p in osteoporosis is unclear. We want to study the mechanism of miR-582-3p on osteogenic differentiation.

Method

The expression of miR-582-3p and homeobox (Hox) A10 were analyzed by quantitative RT-PCR. The expression levels of HOXA10 protein were determined by Western blot. The target of HOXA10 was identified by bioinformatics and luciferase reporter gene assay.

Results

The results showed that miR-582-3p was up-regulated in OP tissues and down-regulated in osteogenic differentiated C2C12 cells compared with that in the control group. Overexpression of miR-582-3p resulted in reduced expression levels of osteocalcin (OC), alkaline phosphatase (ALP), and collagen, type I, α1 (COL1A1). miR-582-3p had a potential binding site with HOXA10. Moreover, miR-582-3p inhibited the expression of HOXA10, and overexpression of HOXA10 reduced the effect of miR-582-3p on osteoblast markers. HOXA10 was the target gene of miR-582-3p, which could inhibit the expression of HOXA10. Furthermore, HOXA10 reduced the role of miR-582-3p in osteoblast markers. miR-582-3p inhibited the development of osteoporosis by regulating HOXA10 and osteoblast differentiation.

Conclusions

miR-582-3p may be a therapeutic target of osteoporosis treatment.

Keywords:

• miRNA-582-3p
• osteogenic differentiation
• HOXA10
• osteoporosis

Ethics approval and consent to participate

This study passed the review board of Ethics Committee of Xinjiang Uygur Autonomous Region People's Hospital. Informed consent was obtained from all individual participants included in the study.

Disclosure statement

The authors declare that they have no competing interests. All authors have read and approved the final article. JY: study design, experiments studies, data analysis and writing the manuscript. JW, WX, QZ, and JS: experimental studies, data analysis and literature research. WenZ: help explain results, artworks. WeiZ: guarantor of integrity of the entire study, literature research, research management and research design.

Data availability statement

The data that support the findings of this study are available on request from the corresponding author.